TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Trautwein-Schult, Anke A1 - Jankowska, Dagmara A1 - Cordes, Arno A1 - Hoferichter, Petra A1 - Klein, Christina A1 - Matros, Andrea A1 - Mock, Hans-Peter A1 - Baronian, Keith A1 - Bode, RĂ¼diger A1 - Kunze, Gotthard T1 - Arxula adeninivorans Recombinant Urate Oxidase and Its Application in the Production of Food with Low Uric Acid Content JF - Journal of Molecular Microbiology and Biotechnology N2 - Hyperuricemia and its symptoms are becoming increasingly common worldwide. Elevated serum uric acid levels are caused by increased uric acid synthesis from food constituents and reduced renal excretion. Treatment in most cases involves reducing alcohol intake and consumption of meat and fish or treatment with pharmaceuticals. Another approach could be to reduce uric acid level in food, either during production or consumption. This work reports the production of recombinant urate oxidase by Arxula adeninivorans and its application to reduce uric acid in a food product. The A. adeninivorans urate oxidase amino acid sequence was found to be similar to urate oxidases from other fungi (61-65% identity). In media supplemented with adenine, hypoxanthine or uric acid, induction of the urate oxidase (AUOX) gene and intracellular accumulation of urate oxidase (Auoxp) was observed. The enzyme characteristics were analyzed from isolates of the wild-type strain A. adeninivorans LS3, as well as from those of transgenic strains expressing the AUOX gene under control of the strong constitutive TEF1 promoter or the inducible AYNI1 promoter. The enzyme showed high substrate specificity for uric acid, a broad temperature and pH range, high thermostability and the ability to reduce uric acid content in food. KW - - KW - Food KW - Hyperuricemia KW - Urate oxidase KW - Uric acid UN - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-31164 UR - www.karger.com/mmb SN - 1464-1801 SS - 1464-1801 SN - 1660-2412 SS - 1660-2412 U6 - https://doi.org/10.1159/000353847 DO - https://doi.org/10.1159/000353847 PM - 24022585 VL - 23 IS - 6 SP - 418 EP - 430 PB - S. Karger AG CY - Basel, Switzerland ER -