TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Homeier-Bachmann, Timo
A1  - Schütz, Anne K.
A1  - Dreyer, Sylvia
A1  - Glanz, Julien
A1  - Schaufler, Katharina
A1  - Conraths, Franz J.
ED  - Oliveira, Manuela
T1  - Genomic Analysis of ESBL-Producing E. coli in Wildlife from North-Eastern Germany
JF  - Antibiotics
N2  - Antimicrobial resistance (AMR) is a serious global health threat and extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales are a major contributor. This study aimed to gain a deeper insight into the AMR burden of wild animals. In total, 1595 fecal samples were collected by two systematic searches in Mecklenburg-Western Pomerania, north-east Germany. Samples were screened for ESBL-carrying Escherichia (E.) coli and isolates found were further analyzed using antimicrobial susceptibility testing and whole-genome sequencing. We found an estimated prevalence of 1.2% ESBL-producing E. coli in wild boar and 1.1% in wild ruminants. CTX-M-1 was the most abundant CTX-M type. We also examined fecal samples from wild boar and wild ruminants using shotgun metagenomics to gain insight into the resistome in wild animals. The latter revealed significantly lower normalized counts for AMR genes in wildlife samples compared to farm animals. The AMR gene levels were lower in wild ruminants than in wild boar. In conclusion, our study revealed a low prevalence of ESBL-producing E. coli and a low overall AMR gene burden in wild boar and wild ruminants, probably due to the secluded location of the search area.
KW  - -
KW  - ESBL-
KW  - wild boar
KW  - wild ruminant
KW  - wildlife
KW  - AMR
KW  - resistome
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-59332
SN  - 2079-6382
SS  - 2079-6382
U6  - https://doi.org/10.3390/antibiotics11020123
DO  - https://doi.org/10.3390/antibiotics11020123
VL  - 11
SP  - 15
S1  - 15
PB  - MDPI
CY  - Basel
ER  -