TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Grambow, Felix
A1  - Rutkowski, Rico
A1  - Podmelle, Fred
A1  - Schmoeckel, Katrin
A1  - Siegerist, Florian
A1  - Domanski, Grzegorz
A1  - Schuster, Matthias W.
A1  - Domanska, Grazyna
T1  - The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer
JF  - International Journal of Molecular Sciences
N2  - The local anesthetic lidocaine, which has been used extensively during liposuction, has been
reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer.
We evaluated the effect of lidocaine on the distribution, number, and viability of adipose-derived stem
cells (ASCs), preadipocytes, mature adipocytes, and leukocytes in the fatty and fluid portion of the
lipoaspirate using antibody staining and flow cytometry analyses. Adipose tissue was harvested from
11 female patients who underwent liposuction. Abdominal subcutaneous fat tissue was infiltrated
with tumescent local anesthesia, containing lidocaine on the left and lacking lidocaine on the right
side of the abdomen, and harvested subsequently. Lidocaine had no influence on the relative
distribution, cell number, or viability of ASCs, preadipocytes, mature adipocytes, or leukocytes in the
stromal-vascular fraction. Assessing the fatty and fluid portions of the lipoaspirate, the fatty portions
contained significantly more ASCs (p < 0.05), stem cells expressing the preadipocyte marker Pref-1
(p < 0.01 w/lidocaine, p < 0.05 w/o lidocaine), and mature adipocytes (p < 0.05 w/lidocaine, p < 0.01
w/o lidocaine) than the fluid portions. Only the fatty portion should be used for transplantation. This
study found no evidence that would contraindicate the use of lidocaine in lipotransfer. Limitations of
the study include the small sample size and the inclusion of only female patients.
KW  - -
KW  - lipofilling
KW  - autologous lipotransfer
KW  - adipose-derived stem cells
KW  - lidocaine
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-65184
SN  - 1422-0067
SS  - 1422-0067
U6  - https://doi.org/10.3390/ijms21082869
DO  - https://doi.org/10.3390/ijms21082869
VL  - 21
IS  - 8
SP  - 19
S1  - 19
PB  - MDPI
CY  - Basel
ER  -