TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Nicolai, Oliver
A1  - Pötschke, Christian
A1  - Schmoeckel, Katrin
A1  - Darisipudi, Murthy N.
A1  - van der Linde, Julia
A1  - Raafat, Dina
A1  - Bröker, Barbara M.
T1  - Antibody Production in Murine Polymicrobial Sepsis—Kinetics and Key Players
JF  - Frontiers in Immunology
N2  - Although antigen-specific priming of antibody responses is impaired during sepsis, there is nevertheless a strong increase in IgM and IgG serum concentrations. Using colon ascendens stent peritonitis (CASP), a mouse model of polymicrobial abdominal sepsis, we observed substantial increases in IgM as well as IgG of all subclasses, starting at day 3 and peaking 2 weeks after sepsis induction. The dominant source of antibody-secreting cells was by far the spleen, with a minor contribution of the mesenteric lymph nodes. Remarkably, sepsis induction in splenectomized mice did not change the dynamics of the serum IgM/IgG reaction, indicating that the marginal zone B cells, which almost exclusively reside in the spleen, are dispensable in such a setting. Hence, in systemic bacterial infection, the function of the spleen as dominant niche of antibody-producing cells can be compensated by extra-splenic B cell populations as well as other lymphoid organs. Depletion of CD4+ T cells did not affect the IgM response, while it impaired IgG generation of all subclasses with the exception of IgG3. Taken together, our data demonstrate that the robust class-switched antibody response in sepsis encompasses both T cell-dependent and -independent components.
KW  - -
KW  - sepsis
KW  - splenectomy
KW  - T cell
KW  - antibody-secreting cells
KW  - IgM
KW  - IgG
Y1  - 2020
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-36805
SN  - 1664-3224
SS  - 1664-3224
U6  - https://doi.org/10.3389/fimmu.2020.00828
DO  - https://doi.org/10.3389/fimmu.2020.00828
VL  - 11
PB  - Frontiers Media S.A.
ER  -