@article{MuellerLangerSiebertetal.2013,
  author    = {G. M{\"u}ller and J. Langer and J. Siebert and A. Kramer},
  title     = {Residual Antimicrobial Effect of Chlorhexidine Digluconate and Octenidine Dihydrochloride on Reconstructed Human Epidermis},
  series   = {Skin Pharmacology and Physiology},
  volume    = {27},
  number    = {1},
  publisher = {S. Karger AG},
  address   = {Basel, Switzerland},
  issn      = {1660-5527},
  doi       = {10.1159/000350172},
  url       = {https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-30785},
  pages     = {1 -- 8},
  year      = {2013},
  abstract  = {The objective of the present investigation was to examine the residual antimicrobial activity after a topical exposure of reconstructed human epidermis (RHE) to equimolar solutions of either chlorhexidine digluconate (CHG, 0.144\% w/v) or octenidine dihydrochloride (OCT, 0.1\% w/v) for 15 min. RHE-associated antiseptic agents were more effective on Staphylococcus aureus than on Pseudomonas aeruginosa. S. aureus was not detected after 24 h of contact, which demonstrated a microbicidal efficacy of greater than 5-log<sub>10</sub> reduction. In contrast, P. aeruginosa was reduced by approximately 2 log<sub>10</sub> at the same incubation time, which parallels the growth of the initial inoculum. This result could be interpreted either as a microbiostatic effect or as an adherence of P. aeruginosa to a low positively charged surface. Small amounts of CHG and OCT can penetrate the stratum corneum. Using these antiseptic agents, the viability of keratinocytes was reduced to 65-75\% of that of the untreated RHE control following 24 h incubation in the presence of test microorganisms. With consideration of antimicrobial activity and cytotoxic effect, OCT corresponds better to a biocompatible antiseptic agent than CHG.},
  language  = {en}
}