TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Schulz, Daniel
A1  - Grumann, Dorothee
A1  - Trübe, Patricia
A1  - Pritchett-Corning, Kathleen
A1  - Johnson, Sarah
A1  - Reppschläger, Kevin
A1  - Gumz, Janine
A1  - Sundaramoorthy, Nandakumar
A1  - Michalik, Stephan
A1  - Berg, Sabine
A1  - van den Brandt, Jens
A1  - Fister, Richard
A1  - Monecke, Stefan
A1  - Uy, Benedict
A1  - Schmidt, Frank
A1  - Bröker, Barbara M.
A1  - Wiles, Siouxsie
A1  - Holtfreter, Silva
T1  - Laboratory Mice Are Frequently Colonized with Staphylococcus aureus and Mount a Systemic Immune Response—Note of Caution for In vivo Infection Experiments
N2  - Whether mice are an appropriate model for S. aureus infection and vaccination studies is a matter of debate, because they are not considered as natural hosts of S. aureus. We previously identified a mouse-adapted S. aureus strain, which caused infections in laboratory mice. This raised the question whether laboratory mice are commonly colonized with S. aureus and whether this might impact on infection experiments. Publicly available health reports from commercial vendors revealed that S. aureus colonization is rather frequent, with rates as high as 21% among specific-pathogen-free mice. In animal facilities, S. aureus was readily transmitted from parents to offspring, which became persistently colonized. Among 99 murine S. aureus isolates from Charles River Laboratories half belonged to the lineage CC88 (54.5%), followed by CC15, CC5, CC188, and CC8. A comparison of human and murine S. aureus isolates revealed features of host adaptation. In detail, murine strains lacked hlb-converting phages and superantigen-encoding mobile genetic elements, and were frequently ampicillin-sensitive. Moreover, murine CC88 isolates coagulated mouse plasma faster than human CC88 isolates. Importantly, S. aureus colonization clearly primed the murine immune system, inducing a systemic IgG response specific for numerous S. aureus proteins, including several vaccine candidates. Phospholipase C emerged as a promising test antigen for monitoring S. aureus colonization in laboratory mice. In conclusion, laboratory mice are natural hosts of S. aureus and therefore, could provide better infection models than previously assumed. Pre-exposure to the bacteria is a possible confounder in S. aureus infection and vaccination studies and should be monitored.
KW  - -
KW  - colonization
KW  - antibody
KW  - laboratory mice
KW  - host adaptation
KW  - genotype
KW  - CC88
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-30138
SN  - 2235-2988
SS  - 2235-2988
U6  - https://doi.org/10.3389/fcimb.2017.00152
DO  - https://doi.org/10.3389/fcimb.2017.00152
VL  - 7
PB  - Frontiers Media S.A.
ER  -