TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Lilge, Lars
A1  - Reder, Alexander
A1  - Tippmann, Frank
A1  - Morgenroth, Friedrich
A1  - Grohmann, Janice
A1  - Becher, Dörte
A1  - Riedel, Katharina
A1  - Völker, Uwe
A1  - Hecker, Michael
A1  - Gerth, Ulf
T1  - The Involvement of the McsB Arginine Kinase in Clp-Dependent Degradation of the MgsR Regulator in Bacillus subtilis
JF  - Frontiers in Microbiology
N2  - Regulated ATP-dependent proteolysis is a common feature of developmental processes and plays also a crucial role during environmental perturbations such as stress and starvation. The Bacillus subtilis MgsR regulator controls a subregulon within the stress- and stationary phase σB regulon. After ethanol exposition and a short time-window of activity, MgsR is ClpXP-dependently degraded with a half-life of approximately 6 min. Surprisingly, a protein interaction analysis with MgsR revealed an association with the McsB arginine kinase and an in vivo degradation assay confirmed a strong impact of McsB on MgsR degradation. In vitro phosphorylation experiments with arginine (R) by lysine (K) substitutions in McsB and its activator McsA unraveled all R residues, which are essentially needed for the arginine kinase reaction. Subsequently, site directed mutagenesis of the MgsR substrate was used to substitute all arginine residues with glutamate (R-E) to mimic arginine phosphorylation and to test their influence on MgsR degradation in vivo. It turned out, that especially the R33E and R94/95E residues (RRPI motif), the latter are adjacently located to the two redox-sensitive cysteines in a 3D model, have the potential to accelerate MgsR degradation. These results imply that selective arginine phosphorylation may have favorable effects for Clp dependent degradation of short-living regulatory proteins. We speculate that in addition to its kinase activity and adaptor function for the ClpC ATPase, McsB might also serve as a proteolytic adaptor for the ClpX ATPase in the degradation mechanism of MgsR.
KW  - -
KW  - Clp proteolysis
KW  - McsB arginine kinase
KW  - MgsR degradation
KW  - arginine phosphorylation
KW  - MgsR activity
Y1  - 2020
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-37621
SN  - 1664-302X
SS  - 1664-302X
U6  - https://doi.org/10.3389/fmicb.2020.00900
DO  - https://doi.org/10.3389/fmicb.2020.00900
VL  - 11
PB  - Frontiers Media S.A.
ER  -