TY  - THES
U1  - Dissertation / Habilitation
A1  - Hildebrandt, Petra
T1  - A highly stereoselective recombinant alcohol dehydrogenase aus 'Pseudomonas fluorescens' DSM50106
N2  - The alcohol dehydrogenase was biochemically characterized. A broad range of arylaliphatic ketones is efficiently reduced to the corresponding optically active (R)-alcohols by a recombinant alcohol dehydrogenase (PF-ADH) produced by overexpression in 'Escherichia coli'. PF-ADH shows high activity and stereoselectivity in the reduction of acetophenone and various derivatives (45-99%), as well as in the reduction of 3-oxy-butyric acid methyl ester and 3-oxy-butyric acid methyl ester and 3-oxy-hexanoic acid ethyl ester (>99%). The highest activity was observed between 10 and 20°C. The copfactor NADH can be efficiently recycled by the addition of 10-20% of iso-propanol. A flow-through-polarimetry-based assay to determine oxidoreductase activity and stereoselectivity is described.
KW  - Oxocarbonsäureester
KW  - Oxidoreductase
KW  - Ketoreductase
KW  - alcohol dehydrogenase
KW  - enantioselectivity
KW  - recombinant enzyme
Y2  - 2005
U6  - https://nbn-resolving.org/urn:nbn:de:gbv:9-000016-5
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-000016-5
ER  -