@phdthesis{Gredes2010,
  author    = {Tomasz Gredes},
  title     = {Changes in insulin like growth factors, myostatin and vascular endothilial growth factor in rat musculus latissimus dorsi by poly-3-hydroxybutyrate implants.},
  journal    = {Changes in insulin like growth factors, myostatin and vascular endothilial growth factor in rat musculus latissimus dorsi by poly-3-hydroxybutyrate implants.},
  url       = {https://nbn-resolving.org/urn:nbn:de:gbv:9-000772-3},
  year      = {2010},
  abstract  = {Bone defects can be a limiting factor in achievement of optimal orthodontic treatment. Diverse bone substitutes were already used for the creation of new bone in the patient, such as collagen composites, calcium phosphate, and titanium. Muscles are known to have a considerable potential of adaptation. The latissimus dorsi muscle was used for the growth and preparation of bone grafts for subsequent transplantation. The aim of the present study was to identify the synergistic effect between the poly-3-hydroxybutyrate (PHB) bone substitute and surrounding muscle tissue. To describe this effect, changes of insulin like growth factors (IGF1, IGF2), myostatin (GDF8) and vascular endothelial growth factor (VEGF) mRNA content were examined in the Musculus latissimus dorsi of 12 Wistar-King rats after 6 and 12 weeks of PHB scaffold implantation. At each time point six rats were killed and implants and surrounding tissues prepared for genetic evaluation. Eight rats without any implants served as controls. RNA was extracted from homogenized muscle tissue and reverse transcribed. Changes in the mRNA content were measured by Real-Time RT-PCR using specific primers for IGF1, IGF2, GDF8, and VEGF. The VEGF mRNA level was significantly increased (p<0.05) in muscle tissue specimens after 6 and 12 weeks of implantation compared to the controls. The expression of the IGF1 gene was also significantly increased as compared to the controls over the observed time period (p<0.05). Furthermore, the VEGF and IGF1 mRNA levels were higher after 6 than after 12 weeks of treatment. In the case of the IGF2 gene, the expression was significantly elevated after 6 weeks (p<0.05), but not significantly increased after 12 weeks. In contrast to all other tested genes we observed a significantly decreased GDF8 gene expression (p<0.05) both after retrieval of implants after 6 as well as after 12 weeks. Moreover, the mRNA levels of GDF8 after 6 and 12 weeks were comparable the same. Our results show that PHB implants in rat Musculus latissimus dorsi interact with the surrounding muscle tissue and have influence on factors that regulate vascularization and muscle adaptation. These findings indicate on one hand a normal wound healing and on the other hand improved muscle regeneration, stating that there is a synergistic effect between PHB scaffolds and the surrounding muscle tissue.},
  language  = {de}
}