@phdthesis{Last2016, author = {Daniel Last}, title = {Novel methods and tools for lactonases, acylases and proteases}, journal = {Neue Methoden und Werkzeuge f{\"u}r Laktonasen, Acylasen und Proteasen}, url = {https://nbn-resolving.org/urn:nbn:de:gbv:9-002642-4}, year = {2016}, abstract = {This thesis is about the establishment and the application of novel methods and tools that are re-lated to the most widely used enzyme class: hydrolases. It covers all fields from the identification to the application of these valuable enzymes with particular focus on lactonases, acylases and proteases. The activity assay introduced in Article I substantially extends the method toolbox for studies on lactonases and acylases that interfere with the bacterial cell-cell communication system. Article II describes a fully automatized robotic platform that represents the next-level tool for the high-throughput enzyme screening in the microtiter plate format. It was used, for instance, for the screening for improved porcine aminoacylase I variants. Diverse aspects of the protease-mediated hydrolysis of non-resistant proteins for the purification of resistant target proteins are highlighted in Article III.}, language = {en} }