@phdthesis{Moche2016,
  author    = {Martin Moche},
  title     = {Optimization and application of the biotinylation approach for extraction and mass-spectrometric analysis of cell-surface associated proteins from Gram-positive bacteria},
  journal    = {Optimierung und Anwendung des Biotinylierungsansatzes f{\"u}r die Extraktion und massenspektrometrische Analyse von zelloberfl{\"a}chenassiziierten Proteinen Gram-positiver Bakterien},
  url       = {https://nbn-resolving.org/urn:nbn:de:gbv:9-002570-4},
  year      = {2016},
  abstract  = {A method employing labeling of cell-surface proteins with Sulfo-NHS-SS-biotin and subsequent affinity enrichment with NeutrAvidin has been optimized in order to make cell-surface proteins from Gram-positive bacteria reliably accessible to quantitative mass spectrometric analyses. The optimized biotinylation approach was applied for analysis of the lipoproteome from S. aureus and S. pneumoniae on a global scale and the influence of mutations in the lipoprotein maturation pathway on the cell-surface and exoproteomes of both species was investigated. The biotinylation approach was integrated into a proteomic workflow that employs metabolic labeling with heavy nitrogen for relative protein quantification to investigate proteomic differences between S. aureus in a biofilm model and its free-floating, planktonic counterparts.},
  language  = {en}
}