@phdthesis{Hildebrandt2006,
  author    = {Petra Hildebrandt},
  title     = {A highly stereoselective recombinant alcohol dehydrogenase aus 'Pseudomonas fluorescens' DSM50106},
  journal    = {Eine hochgradig stereoselektive rekombinante Alkoholdehydrogenase aus 'Pseudomonas fluorescens' DSM50106},
  url       = {https://nbn-resolving.org/urn:nbn:de:gbv:9-000016-5},
  year      = {2006},
  abstract  = {The alcohol dehydrogenase was biochemically characterized. A broad range of arylaliphatic ketones is efficiently reduced to the corresponding optically active (R)-alcohols by a recombinant alcohol dehydrogenase (PF-ADH) produced by overexpression in 'Escherichia coli'. PF-ADH shows high activity and stereoselectivity in the reduction of acetophenone and various derivatives (45-99\%), as well as in the reduction of 3-oxy-butyric acid methyl ester and 3-oxy-butyric acid methyl ester and 3-oxy-hexanoic acid ethyl ester (>99\%). The highest activity was observed between 10 and 20°C. The copfactor NADH can be efficiently recycled by the addition of 10-20\% of iso-propanol. A flow-through-polarimetry-based assay to determine oxidoreductase activity and stereoselectivity is described.},
  language  = {en}
}