@phdthesis{Mallin2014,
  author    = {Hendrik Mallin},
  title     = {Development of Enzymes for Biocatalytic Applications: Protein Engineering, Immobilization and Reactor Concepts},
  journal    = {Entwicklung von Enzymen f{\"u}r den biokatalytischen Einsatz: Proteindesign, Immobilisierung und Reaktor Konzepte},
  url       = {https://nbn-resolving.org/urn:nbn:de:gbv:9-002070-3},
  year      = {2014},
  abstract  = {Within this thesis the protein engineering, immobilization and application of enzymes in organic synthesis were studied in order to enhance the productivity of diverse biotransformations. Article I is a review about Baeyer-Villiger monooxygenases (BVMO) and provides a detailed overview of the most recent advantages in the application of that enzyme class in biocatalysis. Protein engineering of a former uncharacterized polyol-dehydrogenase (PDH) identified in the mesothermophilic bacterium Deinococcus geothermalis 11300 is described in Article II. Article III covers the combination of one PDH mutant with a BVMO in a closed-loop cascade reaction, thus enabling direct oxidation of cyclohexanol to ε-caprolactone with an internal cofactor recycling of NADP(H). Article IV and Article V report a process optimization for transamination reactions due to a newly developed immobilization protocol for five (S)- and (R)-selective aminotransferases (ATA) on chitosan support. Furthermore, the immobilized ATAs were applied in asymmetric amine synthesis. In Article VI, an ATA immobilized on chitosan, an encapsulated BVMO whole cell catalyst and a commercially available immobilized lipase were applied in a traditional fixed-bed (FBR) or stirred-tank reactor (STR), and were compared to a novel reactor design (SpinChem, SCR) for heterogeneous biocatalysis.},
  language  = {en}
}