TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Bekeschus, Sander
A1  - Lackmann, Jan-Wilm
A1  - Gümbel, Denis
A1  - Napp, Matthias
A1  - Schmidt, Anke
A1  - Wende, Kristian
T1  - A Neutrophil Proteomic Signature in Surgical Trauma Wounds
JF  - International Journal of Molecular Sciences
N2  - on-healing wounds continue to be a clinical challenge for patients and medical staff.
These wounds have a heterogeneous etiology, including diabetes and surgical trauma wounds. It is
therefore important to decipher molecular signatures that reflect the macroscopic process of wound
healing. To this end, we collected wound sponge dressings routinely used in vacuum assisted therapy
after surgical trauma to generate wound-derived protein profiles via global mass spectrometry.
We confidently identified 311 proteins in exudates. Among them were expected targets belonging to
the immunoglobulin superfamily, complement, and skin-derived proteins, such as keratins. Next to
several S100 proteins, chaperones, heat shock proteins, and immune modulators, the exudates
presented a number of redox proteins as well as a discrete neutrophil proteomic signature, including
for example cathepsin G, elastase, myeloperoxidase, CD66c, and lipocalin 2. We mapped over 200
post-translational modifications (PTMs; cysteine/methionine oxidation, tyrosine nitration, cysteine
trioxidation) to the proteomic profile, for example, in peroxiredoxin 1. Investigating manually
collected exudates, we confirmed presence of neutrophils and their products, such as microparticles
and fragments containing myeloperoxidase and DNA. These data confirmed known and identified
less known wound proteins and their PTMs, which may serve as resource for future studies on
human wound healing
KW  - -
KW  - chaperones
KW  - damage-associated molecular patterns
KW  - heat-shock proteins
KW  - mass spectrometry
KW  - matrix metalloproteinase
KW  - peptidases
KW  - post-translational modifications
KW  - redox regulation
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-65267
SN  - 1422-0067
SS  - 1422-0067
U6  - https://doi.org/10.3390/ijms19030761
DO  - https://doi.org/10.3390/ijms19030761
VL  - 19
IS  - 3
SP  - 23
S1  - 23
PB  - MDPI
CY  - Basel
ER  -