TY - THES U1 - Dissertation / Habilitation A1 - Nawka, Mato T1 - Isolierung und Charakterisierung eines rekombinanten Nucleophosmins und Nachweis von Nucleophosmin als Bestandteil fructoselysinspezifischer Rezeptoren in Zellmembranen der monocytären Zelllinien Mono Mac 6 und U937 N2 - keine Angabe N2 - Biochim Biophys Acta. 2004 Jan 22;1670(2):132-6. Nucleophosmin is a component of the fructoselysine-specific receptor in cell membranes of Mono Mac 6 and U937 monocyte-like cells. Brandt R, Nawka M, Kellermann J, Salazar R, Becher D, Krantz S. Institute of Medical Biochemistry and Molecular Biology, Ernst Moritz Arndt University, Klinikum, Sauerbruchstrasse, D-17487 Greifswald, Germany. The monocyte-like cell lines Mono Mac 6 (MM6) and U937 bind Amadori-modified proteins via fructoselysine (FL)-specific sites with molar masses of 110, 150 and 200 kDa, which can specifically be isolated by an affinity method with magnetobeads coated with glycated polylysine. Using Western blots developed with different anti-nucleophosmin antisera, MS-analysis and immunohistochemistry, we show that the nucleolar protein nucleophosmin is also localized in the cell membrane and is part of the 150- and 200-kDa membrane protein fractions of FL-specific binding membrane proteins. This is the first evidence that nucleophosmin is not only existing in the nucleolus and cytoplasm, but also, like nucleolin, is in the cell membrane. KW - Glykation KW - Fructoselysin KW - Rezeptor KW - Monozyt KW - Zelllinie KW - Nukleophosmin KW - nucleophosmin Y2 - 2005 U6 - https://nbn-resolving.org/urn:nbn:de:gbv:9-000335-2 UN - https://nbn-resolving.org/urn:nbn:de:gbv:9-000335-2 ER -