@phdthesis{Laporte2019,
  author    = {Anna Laporte},
  title     = {Site-specific intracellular generation and detection of hydrogen peroxide and protection against oxidative stress in insulin-producing cells},
  journal    = {Kompartimentspezifische intrazellul{\"a}re Bildung und Detektion von Wasserstoffperoxid sowie Schutz gegen{\"u}ber oxidativem Stress in insulinproduzierenden Zellen},
  url       = {https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-31847},
  pages     = {226},
  year      = {2019},
  abstract  = {The term diabetes mellitus comprises a group of metabolic diseases all distinguished by their main characteristic hyperglycaemia. With a steadily increasing prevalence diabetes displays an enormous burden for patients and health systems and is therefore of special interest for research. The development of the two main types of diabetes, type 1 and type 2, is closely linked to the formation of reactive species, especially hydrogen peroxide, inside different compartments of pancreatic beta cells. However, these cells are especially vulnerable towards oxidative stress mediated by hydrogen peroxide due to a low expression of antioxidative enzymes. The main aims of the present thesis were to analyse the intracellular generation and to enable the site-specific detection of hydrogen peroxide to evaluate its role in the delicate equilibrium between redox signalling and oxidative stress under certain pathophysiological conditions, and moreover to monitor its movement through compartments and subcellular membranes of insulin-producing cells. Additionally, a new methodology for an artificial site-specific generation of hydrogen peroxide inside living cells was developed.},
  language  = {en}
}