TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Müller, G.
A1  - Langer, J.
A1  - Siebert, J.
A1  - Kramer, A.
T1  - Residual Antimicrobial Effect of Chlorhexidine Digluconate and Octenidine Dihydrochloride on Reconstructed Human Epidermis
JF  - Skin Pharmacology and Physiology
N2  - The objective of the present investigation was to examine the residual antimicrobial activity after a topical exposure of reconstructed human epidermis (RHE) to equimolar solutions of either chlorhexidine digluconate (CHG, 0.144% w/v) or octenidine dihydrochloride (OCT, 0.1% w/v) for 15 min. RHE-associated antiseptic agents were more effective on Staphylococcus aureus than on Pseudomonas aeruginosa. S. aureus was not detected after 24 h of contact, which demonstrated a microbicidal efficacy of greater than 5-log<sub>10</sub> reduction. In contrast, P. aeruginosa was reduced by approximately 2 log<sub>10</sub> at the same incubation time, which parallels the growth of the initial inoculum. This result could be interpreted either as a microbiostatic effect or as an adherence of P. aeruginosa to a low positively charged surface. Small amounts of CHG and OCT can penetrate the stratum corneum. Using these antiseptic agents, the viability of keratinocytes was reduced to 65-75% of that of the untreated RHE control following 24 h incubation in the presence of test microorganisms. With consideration of antimicrobial activity and cytotoxic effect, OCT corresponds better to a biocompatible antiseptic agent than CHG.
KW  - -
KW  - Octenidine dihydrochloride
KW  - Reconstructed human epidermis
KW  - Antiseptics
KW  - Chlorhexidine digluconate
KW  - Residual antimicrobial effect
Y1  - 2013
UN  - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-30785
SN  - 1660-5527
SS  - 1660-5527
SN  - 1660-5535
SS  - 1660-5535
U6  - https://doi.org/10.1159/000350172
DO  - https://doi.org/10.1159/000350172
PM  - 23887383
VL  - 27
IS  - 1
SP  - 1
EP  - 8
PB  - S. Karger AG
CY  - Basel, Switzerland
ER  -