TY - JOUR U1 - Wissenschaftlicher Artikel A1 - Lilge, Lars A1 - Reder, Alexander A1 - Tippmann, Frank A1 - Morgenroth, Friedrich A1 - Grohmann, Janice A1 - Becher, Dörte A1 - Riedel, Katharina A1 - Völker, Uwe A1 - Hecker, Michael A1 - Gerth, Ulf T1 - The Involvement of the McsB Arginine Kinase in Clp-Dependent Degradation of the MgsR Regulator in Bacillus subtilis JF - Frontiers in Microbiology N2 - Regulated ATP-dependent proteolysis is a common feature of developmental processes and plays also a crucial role during environmental perturbations such as stress and starvation. The Bacillus subtilis MgsR regulator controls a subregulon within the stress- and stationary phase σB regulon. After ethanol exposition and a short time-window of activity, MgsR is ClpXP-dependently degraded with a half-life of approximately 6 min. Surprisingly, a protein interaction analysis with MgsR revealed an association with the McsB arginine kinase and an in vivo degradation assay confirmed a strong impact of McsB on MgsR degradation. In vitro phosphorylation experiments with arginine (R) by lysine (K) substitutions in McsB and its activator McsA unraveled all R residues, which are essentially needed for the arginine kinase reaction. Subsequently, site directed mutagenesis of the MgsR substrate was used to substitute all arginine residues with glutamate (R-E) to mimic arginine phosphorylation and to test their influence on MgsR degradation in vivo. It turned out, that especially the R33E and R94/95E residues (RRPI motif), the latter are adjacently located to the two redox-sensitive cysteines in a 3D model, have the potential to accelerate MgsR degradation. These results imply that selective arginine phosphorylation may have favorable effects for Clp dependent degradation of short-living regulatory proteins. We speculate that in addition to its kinase activity and adaptor function for the ClpC ATPase, McsB might also serve as a proteolytic adaptor for the ClpX ATPase in the degradation mechanism of MgsR. KW - - KW - Clp proteolysis KW - McsB arginine kinase KW - MgsR degradation KW - arginine phosphorylation KW - MgsR activity Y1 - 2020 UN - https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-37621 SN - 1664-302X SS - 1664-302X U6 - https://doi.org/10.3389/fmicb.2020.00900 DO - https://doi.org/10.3389/fmicb.2020.00900 VL - 11 PB - Frontiers Media S.A. ER -