Volltext-Downloads (blau) und Frontdoor-Views (grau)
The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 10 of 30
Back to Result List

Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-32059

Mast Cells Are Activated by Streptococcus pneumoniae In Vitro but Dispensable for the Host Defense Against Pneumococcal Central Nervous System Infection In Vivo

  • Mast cells reside on and near the cerebral vasculature, the predominant site of pneumococcal entry into the central nervous system (CNS). Although mast cells have been reported to be crucial in protecting from systemic bacterial infections, their role in bacterial infections of the CNS remained elusive. Here, we assessed the role of mast cells in pneumococcal infection in vitro and in vivo. In introductory experiments using mouse bone marrow-derived mast cells (BMMC), we found that (i) BMMC degranulate and release selected cytokines upon exposure to Streptococcus pneumoniae, (ii) the response of BMMC varies between different pneumococcal serotypes and (iii) is dependent on pneumolysin. Intriguingly though, apart from a slight enhancement of cerebrospinal fluid (CSF) pleocytosis, neither two different mast cell-deficient Kit mutant mouse strains (WBB6F1-KitW/Wv and C57BL/6 KitW-sh/W-sh mice) nor pharmacologic mast cell stabilization with cromoglycate had any significant impact on the disease phenotype of experimental pneumococcal meningitis. The incomplete reversal of the enhanced CSF pleocytosis by local mast cell engraftment suggests that this phenomenon is caused by other c-Kit mutation-related mechanisms than mast cell deficiency. In conclusion, our study suggests that mast cells can be activated by S. pneumoniae in vitro. However, mast cells do not play a significant role as sentinels of pneumococcal CSF invasion and initiators of innate immunity in vivo.

Download full text files

Export metadata

Additional Services

Search Google Scholar

Statistics

frontdoor_oas
Metadaten
Author: Johanna Fritscher, Daniel Amberger, Susanne Dyckhoff, Jan Philipp Bewersdorf, Ilias Masouris, Stefanie Voelk, Sven Hammerschmidt, Helga Maria Schmetzer, Matthias Klein, Hans-Walter Pfister, Uwe Koedel
URN:urn:nbn:de:gbv:9-opus-32059
DOI:https://doi.org/10.3389/fimmu.2018.00550
ISSN:1664-3224
Parent Title (English):Frontiers in Immunology
Publisher:Frontiers Media S.A.
Document Type:Article
Language:English
Date of first Publication:2018/03/19
Release Date:2020/10/05
Tag:bacterial meningitis; innate immunity; mast cell; mouse model
GND Keyword:-
Volume:9
Faculties:Universitätsmedizin / Interfakultäres Institut für Genetik und Funktionelle Genomforschung (UMG)
Licence (German):License LogoUrheberrechtlich geschützt