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Antibody production in murine polymicrobial sepsis – kinetics and key players

  • Although antigen-specific priming of antibody responses isimpaired during sepsis, thereis nevertheless a strong increase in IgM and IgG serum concentrations. Using colonascendens stent peritonitis (CASP), a mouse model of polymicrobial abdominal sepsis,we observed substantial increases in IgM as well as IgG of allsubclasses, starting at day 3and peaking 2 weeks after sepsis induction. The dominant source of antibody-secretingcells was by far the spleen, with a minor contribution of the mesenteric lymph nodes.Remarkably, sepsis induction in splenectomized mice did not change the dynamics ofthe serum IgM/IgG reaction, indicating that the marginal zone B cells, which almostexclusively reside in the spleen, are dispensable in such a setting. Hence, in systemicbacterial infection, the function of the spleen as dominantniche of antibody-producingcells can be compensated by extra-splenic B cell populations as well as other lymphoidorgans. Depletion of CD4+T cells did not affect the IgM response, while it impairedIgG generation of all subclasses with the exception of IgG3.Taken together, our datademonstrate that the robust class-switched antibody response in sepsis encompassesboth T cell-dependent and -independent components

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Metadaten
Author: Oliver Nicolai, Christian Pötschke, Katrin Schmoeckel, Murthy Darisipudi, Julia van der Linde, Dina RaafatORCiD, Barbara Bröker
URN:urn:nbn:de:gbv:9-opus-43965
DOI:https://doi.org/10.3389/fimmu.2020.00828
ISSN:1664-3224
Parent Title (English):Frontiers in Immunology
Publisher:Frontiers
Place of publication:Lausanne
Document Type:Article
Language:English
Date of first Publication:2020/04/30
Release Date:2021/03/15
Tag:IgG, IgM, T cell, antibody-secretingcells, sepsis, splenectomy
Faculties:Universitätsmedizin / Institut für Immunologie u. Transfusionsmedizin - Abteilung Immunologie
Licence (German):License LogoCreative Commons - Namensnennung