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Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates
- Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca2+ release from storage organelles and higher activity of Ca2+ pumps at temperatures < 15 °C triggers cytoskeleton changes. This is suppressed by Mg2+ addition, avoiding a shift in Ca2+ hemostasis and cytoskeletal alterations. We report on the impact of 2–10 mM Mg2+ on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 °C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affected by storage at 4 °C and preserved by Mg2+ addition ≥ 4 mM Mg2+ (mean ± SD of median deformation 4 °C vs. 4 °C + 10 mM Mg2+ 0.073 ± 0.021 vs. 0.118 ± 0.023, p < 0.01; n = 6, day 7). These results were confirmed by immunofluorescence microscopy, showing that Mg2+ ≥ 4 mM prevents 4 °C storage induced cytoskeletal structure lesion. Standard in vitro platelet function tests showed minor differences between RT and cold-stored platelets. Hypotonic shock response was not significantly different between RT stored (56.38 ± 29.36%) and cold-stored platelets with (55.22 ± 11.16%) or without magnesium (45.65 ± 11.59%; p = 0.042, all n = 6, day 1). CD62P expression and platelet aggregation response were similar between RT and 4 °C stored platelets, with minor changes in the presence of higher Mg2+ concentrations. In conclusion, increasing Mg2+ up to 10 mM in PAS counteracts 4 °C storage lesions in platelets, maintains platelet cytoskeletal integrity and biomechanical properties comparable to RT stored platelets.
Author: | Konstanze Aurich, Jan Wesche, Martin Ulbricht, Oliver Otto, Andreas Greinacher, Raghavendra Palankar |
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URN: | urn:nbn:de:gbv:9-opus-104904 |
DOI: | https://doi.org/10.1038/s41598-022-10231-x |
ISSN: | 2045-2322 |
Parent Title (English): | Scientific Reports |
Publisher: | Nature Publishing Group |
Place of publication: | London |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2022/04/14 |
Release Date: | 2024/01/19 |
Volume: | 12 |
Article Number: | 6229 |
Page Number: | 9 |
Faculties: | Universitätsmedizin / Institut für Immunologie u. Transfusionsmedizin - Abteilung Transfusionsmedizin |
Collections: | Artikel aus DFG-gefördertem Publikationsfonds |
Licence (German): | Creative Commons - Namensnennung |