Chemoenzymatic Cascade Reaction for the Valorization of the Lignin Depolymerization Product G−C2-Dioxolane Phenol

Combining solid acid catalysts with enzyme reactions in aqueous environments is challenging because either very acidic conditions inactivate the enzymes, or the solid acid catalyst is neutralized. In this study, Amberlyst-15 encapsulated in polydimethylsiloxane (Amb-15@PDMS) is used to deprotect the lignin depolymerization product G−C2 dioxolane phenol in a buffered system at pH 6.0. This reaction is directly coupled with the biocatalytic reduction of the released homovanillin to homovanillyl alcohol by recombinant horse liver alcohol dehydrogenase, which is subsequently acylated by the promiscuous acyltransferase/hydrolase PestE_I208A_L209F_N288A in a one-pot system. The deprotection catalyzed with Amb-15@PDMS attains up to 97 % conversion. Overall, this cascade enables conversions of up to 57 %.

Metadaten
Author:	Henrik Terholsen, Jule R. H. Meyer, Zhenlei Zhang, Peter J. Deuss, Uwe T. Bornscheuer
URN:	urn:nbn:de:gbv:9-opus-109138
DOI:	https://doi.org/10.1002/cssc.202300168
ISSN:	1864-564X
Parent Title (English):	ChemSusChem
Publisher:	Wiley
Place of publication:	Hoboken, NJ
Document Type:	Article
Language:	English
Date of Publication (online):	2023/02/24
Date of first Publication:	2023/05/19
Release Date:	2024/03/25
Tag:	biocatalysis; biomass valorization; chemoenzymatic cascade; heterogeneous catalysis; lignin
Volume:	16
Issue:	10
Article Number:	e202300168
Page Number:	5
Faculties:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie
Collections:	weitere DFG-förderfähige Artikel
Licence (German):	Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 4.0 International

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