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Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-31164

Arxula adeninivorans Recombinant Urate Oxidase and Its Application in the Production of Food with Low Uric Acid Content

  • Hyperuricemia and its symptoms are becoming increasingly common worldwide. Elevated serum uric acid levels are caused by increased uric acid synthesis from food constituents and reduced renal excretion. Treatment in most cases involves reducing alcohol intake and consumption of meat and fish or treatment with pharmaceuticals. Another approach could be to reduce uric acid level in food, either during production or consumption. This work reports the production of recombinant urate oxidase by Arxula adeninivorans and its application to reduce uric acid in a food product. The A. adeninivorans urate oxidase amino acid sequence was found to be similar to urate oxidases from other fungi (61-65% identity). In media supplemented with adenine, hypoxanthine or uric acid, induction of the urate oxidase (AUOX) gene and intracellular accumulation of urate oxidase (Auoxp) was observed. The enzyme characteristics were analyzed from isolates of the wild-type strain A. adeninivorans LS3, as well as from those of transgenic strains expressing the AUOX gene under control of the strong constitutive TEF1 promoter or the inducible AYNI1 promoter. The enzyme showed high substrate specificity for uric acid, a broad temperature and pH range, high thermostability and the ability to reduce uric acid content in food.

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Metadaten
Author: Anke Trautwein-Schult, Dagmara Jankowska, Arno Cordes, Petra Hoferichter, Christina Klein, Andrea Matros, Hans-Peter Mock, Keith Baronian, Rüdiger Bode, Gotthard Kunze
URN:urn:nbn:de:gbv:9-opus-31164
URL:http://www.karger.com/mmb
DOI:https://doi.org/10.1159/000353847
ISSN:1464-1801
ISSN:1660-2412
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24022585
Parent Title (English):Journal of Molecular Microbiology and Biotechnology
Publisher:S. Karger AG
Place of publication:Basel, Switzerland
Document Type:Article
Language:English
Date of first Publication:2013/09/06
Release Date:2020/09/29
Tag:Food, Hyperuricemia, Urate oxidase, Uric acid
GND Keyword:-
Volume:23
Issue:6
First Page:418
Last Page:430
Faculties:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Mikrobiologie - Abteilung für Genetik & Biochemie
Licence (German):License LogoUrheberrechtlich geschützt