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Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-31861

Purification and proteomics of pathogen-modified vacuoles and membranes

  • Certain pathogenic bacteria adopt an intracellular lifestyle and proliferate in eukaryotic host cells. The intracellular niche protects the bacteria from cellular and humoral components of the mammalian immune system, and at the same time, allows the bacteria to gain access to otherwise restricted nutrient sources. Yet, intracellular protection and access to nutrients comes with a price, i.e., the bacteria need to overcome cell-autonomous defense mechanisms, such as the bactericidal endocytic pathway. While a few bacteria rupture the early phagosome and escape into the host cytoplasm, most intracellular pathogens form a distinct, degradation-resistant and replication-permissive membranous compartment. Intracellular bacteria that form unique pathogen vacuoles include Legionella, Mycobacterium, Chlamydia, Simkania, and Salmonella species. In order to understand the formation of these pathogen niches on a global scale and in a comprehensive and quantitative manner, an inventory of compartment-associated host factors is required. To this end, the intact pathogen compartments need to be isolated, purified and biochemically characterized. Here, we review recent progress on the isolation and purification of pathogen-modified vacuoles and membranes, as well as their proteomic characterization by mass spectrometry and different validation approaches. These studies provide the basis for further investigations on the specific mechanisms of pathogen-driven compartment formation.

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Metadaten
Author: Jo-Ana Herweg, Nicole Hansmeier, Andreas Otto, Anna C. Geffken, Prema Subbarayal, Bhupesh K. Prusty, Dörte Becher, Michael Hensel, Ulrich E. Schaible, Thomas Rudel, Hubert Hilbi
URN:urn:nbn:de:gbv:9-opus-31861
DOI:https://doi.org/10.3389/fcimb.2015.00048
ISSN:2235-2988
Parent Title (English):Frontiers in Cellular and Infection Microbiology
Publisher:Frontiers Media S.A.
Document Type:Article
Language:English
Date of first Publication:2015/06/02
Release Date:2020/10/05
Tag:host-pathogen interactions, immuno-magnetic purification, pathogen vacuole
GND Keyword:-
Volume:5
Faculties:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Mikrobiologie - Abteilung für Genetik & Biochemie
Licence (German):License LogoCreative Commons - Namensnennung