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Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades
- Plant-derived carbohydrates are an abundant renewable re- source. Transformation of carbohydrates into new products, in- cluding amine-functionalized building blocks for biomaterials applications, can lower reliance on fossil resources. Herein, bio- catalytic production routes to amino carbohydrates, including oligosaccharides, are demonstrated. In each case, two-step bio- catalysis was performed to functionalize d-galactose-contain- ing carbohydrates by employing the galactose oxidase from Fusarium graminearum or a pyranose dehydrogenase from Agaricus bisporus followed by the w-transaminase from Chro- mobacterium violaceum (Cvi-w-TA). Formation of 6-amino-6- deoxy-d-galactose, 2-amino-2-deoxy-d-galactose, and 2-amino- 2-deoxy-6-aldo-d-galactose was confirmed by mass spectrome- try. The activity of Cvi-w-TA was highest towards 6-aldo-d-gal- actose, for which the highest yield of 6-amino-6-deoxy-d-galac- tose (67%) was achieved in reactions permitting simultaneous oxidation of d-galactose and transamination of the resulting 6- aldo-d-galactose.
Author: | Prof. Dr. Uwe BornscheuerORCiD, Ville Aumala, Filip Mollerup, Dr. Edita Jurak, Fabian Blume, Dr. Johanna Karppi, Antti E. Koistinen, Eva Schuiten, Moritz Voß, Prof. Jan Deska, Prof. Emma R. Master |
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URN: | urn:nbn:de:gbv:9-opus-60313 |
DOI: | https://doi.org/10.1002/cssc.201802580 |
ISSN: | 1864-564X |
Parent Title (English): | ChemSusChem |
Document Type: | Article |
Language: | English |
Year of Completion: | 2019 |
Release Date: | 2022/03/22 |
Tag: | amination |
GND Keyword: | Enzym |
Volume: | 12 |
Issue: | 4 |
First Page: | 848 |
Last Page: | 857 |
Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie |
DDC class: | 500 Naturwissenschaften und Mathematik / 540 Chemie |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |