Volltext-Downloads (blau) und Frontdoor-Views (grau)
The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 2 of 4
Back to Result List

Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-39995

Poly (hexamethylene biguanide), adsorbed onto Ti‐Al‐V alloys, kills slime‐producing Staphylococci and Pseudomonas aeruginosa without inhibiting SaOs‐2 cell differentiation

  • Abstract Antimicrobial coating of implant material with poly(hexamethylene biguanide) hydrochloride (PHMB) may be an eligible method for preventing implant‐associated infections. In the present study, an antibacterial effective amount of PHMB is adsorbed on the surface of titanium alloy after simple chemical pretreatment. Either oxidation with 5% H2O2 for 24 hr or processing for 2 hr in 5 M NaOH provides the base for the subsequent formation of a relatively stable self‐assembled PHMB layer. Compared with an untreated control group, adsorbed PHMB produces no adverse effects on SaOs‐2 cells within 48 hr cell culture, but promotes the initial attachment and spreading of the osteoblasts within 15 min. Specimens were inoculated with slime‐producing bacteria to simulate a perioperative infection. Adsorbed PHMB reacts bactericidally against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa after surface contact. Adhered SaOs‐2 cells differentiate and produce alkaline phosphatase and deposit calcium within 4 days in a mineralization medium on PHMB‐coated Ti6Al4V surfaces, which have been precontaminated with S. epidermidis. The presented procedures provide a simple method for generating biocompatibly and antimicrobially effective implant surfaces that may be clinically important.

Download full text files

Export metadata

Additional Services

Search Google Scholar

Statistics

frontdoor_oas
Metadaten
Author: Melanie Hornschuh, Paula Zwicker, Thomas Schmidt, Birgit Finke, Axel Kramer, Gerald Müller
URN:urn:nbn:de:gbv:9-opus-39995
DOI:https://doi.org/10.1002/jbm.b.34522
Parent Title (English):Journal of Biomedical Materials Research Part B: Applied Biomaterials
Publisher:John Wiley & Sons, Inc.
Place of publication:Hoboken, USA
Document Type:Article
Language:English
Date of first Publication:2020/04/24
Release Date:2020/12/01
Tag:PHMB adsorption; SaOs‐2 cell differentiation; contact killing surface; surface modification; titanium alloys
GND Keyword:-
Volume:108
Issue:5
First Page:1801
Last Page:1813
Faculties:Universitätsmedizin / Institut für Hygiene und Umweltmedizin
Licence (German):License LogoCreative Commons - Namensnennung