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G‐Quadruplex Formation in a Putative Coding Region of White Spot Syndrome Virus: Structural and Thermodynamic Aspects
- Abstract White spot disease (WSD) is one of the most devastating viral infections of crustaceans caused by the white spot syndrome virus (WSSV). A conserved sequence WSSV131 in the DNA genome of WSSV was found to fold into a polymorphic G‐quadruplex structure. Supported by two mutant sequences with single G→T substitutions in the third G4 tract of WSSV131, circular dichroism and NMR spectroscopic analyses demonstrate folding of the wild‐type sequence into a three‐tetrad parallel topology comprising three propeller loops with a major 1 : 3 : 1 and a minor 1 : 2 : 2 loop length arrangement. A thermodynamic analysis of quadruplex formation by differential scanning calorimetry (DSC) indicates a thermodynamically more stable 1 : 3 : 1 loop isomer. DSC also revealed the formation of additional highly stable multimeric species with populations depending on potassium ion concentration.
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| Author: | Yoanes Maria Vianney, Maria Goretti M. Purwanto, Klaus Weisz |
|---|---|
| URN: | urn:nbn:de:gbv:9-opus-46208 |
| DOI: | https://doi.org/10.1002/cbic.202100064 |
| Parent Title (English): | ChemBioChem |
| Document Type: | Article |
| Language: | English |
| Date of first Publication: | 2021/04/06 |
| Release Date: | 2021/06/28 |
| Tag: | G-quadruplexes; NMR spectroscopy; thermodynamics; white spot syndrome virus |
| GND Keyword: | - |
| First Page: | n/a |
| Last Page: | n/a |
| Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie |
| Licence (German): |  Creative Commons - Namensnennung |