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Hirudin or hirudin‐like factor ‐ that is the question: insights from the analyses of natural and synthetic HLF variants
- The hirudin‐like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech‐derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF‐Hyb, a yet uncharacterized member of the HLF family. Modifications within the N terminus of HLF1 have a strong impact on its activity. Some variants of HLF1 exhibit thrombin‐inhibiting activity comparable to hirudins, whereas others have reduced or no activity. The analyses of HLF‐Hyb variants revealed a strong impact of the central globular domain on activity. Our results indicate a comparable mode of action of hirudins and thrombin‐inhibiting HLF variants. Finally, we propose and discuss criteria for classifying hirudins and HLFs.
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| Author: | Christian Müller, Phil Lukas, Michel Böhmert, Jan‐Peter Hildebrandt |
|---|---|
| URN: | urn:nbn:de:gbv:9-opus-40018 |
| DOI: | https://doi.org/10.1002/1873-3468.13683 |
| Parent Title (English): | FEBS Letters |
| Publisher: | Wiley |
| Place of publication: | Hoboken, New Jersey |
| Document Type: | Article |
| Language: | English |
| Date of first Publication: | 2020/03/08 |
| Release Date: | 2020/12/01 |
| Tag: | Hirudin; Hirudin‐like factors; blood coagulation; medicinal leeches |
| GND Keyword: | - |
| Volume: | 594 |
| Issue: | 5 |
| First Page: | 841 |
| Last Page: | 850 |
| Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell |