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An Enzyme Cascade Reaction for the Recovery of Hydroxytyrosol Dervatives from Olive Mill Wastewater
- Abstract The valorization of olive mill wastewaters (OMWW), a by‐product of the olive milling, is getting rising attention. Lipophilization of the main phenolic compound 3‐hydroxytyrosol (HT) could facilitate its extraction. An immobilized variant of the promiscuous hydrolase/acyltransferase from Pyrobaculum calidifontis VA1 (PestE) was used to perform acetylation in water using ethyl acetate as acyl donor. PestE was used in a segmented flow setting to allow continuous operation. Additionally, HT precursors were made accessible by pretreatment with almond β‐glucosidase and the hydrolytic activity of PestE_I208A_L209F_N288A.
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| Author: | Henrik Terholsen, Jasmin Kaur, Nikolaos Kaloudis, Amanda Staudt, Ioannis V. Pavlidis, Uwe T. BornscheuerORCiD |
|---|---|
| URN: | urn:nbn:de:gbv:9-opus-76656 |
| DOI: | https://doi.org/10.1002/cite.202200069 |
| ISSN: | 1522-2640 |
| Parent Title (English): | Chemie Ingenieur Technik |
| Publisher: | Wiley |
| Place of publication: | Hoboken, NJ |
| Document Type: | Article |
| Language: | English |
| Date of first Publication: | 2022/10/19 |
| Release Date: | 2022/11/29 |
| Tag: | Cascade reaction; Flow catalysis; Hydroxytyrosol; Olive mill wastewaters valorization; Promiscuous acyltransferase |
| Volume: | 94 |
| Issue: | 11 |
| First Page: | 1860 |
| Last Page: | 1863 |
| Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie |
| Collections: | weitere DFG-förderfähige Artikel |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |