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Substrate specificity of thioredoxins and glutaredoxins–towards afunctional classification
- The spatio-temporal reduction and oxidation of protein thiols is an essential mechanism in signal transduction inall kingdoms of life. Thioredoxin (Trx) family proteins efficiently catalyze thiol-disulfide exchange reactions andthe proteins are widely recognized for their importance in the operation of thiol switches. Trx family proteinshave a broad and at the same time very distinct substrate specificity–a prerequisite for redox switching. Despiteof multiple efforts, the true nature for this specificity is still under debate. Here, we comprehensively compare theclassification/clustering of various redoxins from all domains of life based on their similarity in amino acidsequence, tertiary structure, and their electrostatic properties. We correlate these similarities to the existence ofcommon interaction partners, identified in various previous studies and suggested by proteomic screenings. Theseanalyses confirm that primary and tertiary structure similarity, and thereby all common classification systems, donot correlate to the target specificity of the proteins as thiol-disulfide oxidoreductases. Instead, a number ofexamples clearly demonstrate the importance of electrostatic similarity for their target specificity, independent oftheir belonging to the Trx or glutaredoxin subfamilies
Author: | Manuela Gellert, Faruq Hossain, Felix Berens, Lukas Bruhn, Volkmar Urbainsky, Christopher Horst LilligORCiD |
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URN: | urn:nbn:de:gbv:9-opus-43776 |
DOI: | https://doi.org/10.1016/j.heliyon.2019.e02943 |
ISSN: | 2405-8440 |
Parent Title (English): | Heliyon |
Publisher: | Elsevier |
Place of publication: | London |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2019/11/25 |
Release Date: | 2021/03/08 |
Tag: | Biocomputational metho; Biomolecules; Gromov-Wasserstein distance; Mathematical bioscience; Redox signaling; Thioredoxin |
Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Abteilung für Mikrobiologie und Molekularbiologie |
Collections: | Artikel aus DFG-gefördertem Publikationsfonds |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |