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Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-31319

Postoperative Immune Suppression in Visceral Surgery: Characterisation of an Intestinal Mouse Model

  • Background: Postoperatively acquired immune dysfunction is associated with a higher mortality rate in case of septic complications. As details of this severe clinical problem are still unknown, animal models are essential to characterise the mechanisms involved. Methods: Mice were laparotomised and the small intestine was pressed smoothly in antegrade direction. For extension of trauma, the intestine was manipulated three times consecutively. Following this, the ex vivo cytokine release of splenocytes was determined. The degree of surgical trauma was analysed by detection of HMGB1 and IL-6 in serum and by neutrophil staining in the muscularis mucosae. Results: We adapted the previously described animal model of intestinal manipulation to provide a model of surgically induced immune dysfunction. Following intestinal manipulation, the mice showed elevated serum levels of HMGB1 and IL-6 and increased infiltration of granulocytes into the muscularis mucosae. Ex vivo cytokine release by splenocytes was suppressed in the postoperative period. The degree of suppression correlated with the extent of surgical trauma. Conclusions: In this study, we describe a surgically induced immune dysfunction animal model, in which a significant surgical trauma is followed by an immune dysfunction. This model may be ideal for the characterisation of the postoperative immune dysfunction syndrome.

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Metadaten
Author: P. Koerner, A. Busemann, T. Traeger, W. Kessler, K. Cziupka, S. Diedrich, C. Kloecker, R. Jack, C.-D. Heidecke, S. Maier
URN:urn:nbn:de:gbv:9-opus-31319
URL:http://www.karger.com/esr
DOI:https://doi.org/10.1159/000333391
ISSN:0014-312X
ISSN:1421-9921
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=22075937
Parent Title (English):European Surgical Research
Publisher:S. Karger AG
Place of publication:Basel, Switzerland
Document Type:Article
Language:English
Date of first Publication:2011/11/09
Release Date:2020/09/29
Tag:Cytokines, Mouse model, Postoperative immune suppression, Surgery, Surgically induced immune dysfunction
GND Keyword:-
Volume:47
Issue:4
First Page:260
Last Page:266
Faculties:Universit├Ątsmedizin / Institut f├╝r Immunologie u. Transfusionsmedizin - Abteilung Immunologie
Licence (German):License LogoUrheberrechtlich gesch├╝tzt