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Boronic Acid‐Mediated Activity Control of Split 10–23 DNAzymes
- Abstract The 10–23 DNAzyme is an artificially developed Mg2+‐dependent catalytic oligonucleotide that can cleave an RNA substrate in a sequence‐specific fashion. In this study, new split 10–23 DNAzymes made of two nonfunctional fragments, one of which carries a boronic acid group at its 5′ end, while the other has a ribonucleotide at its 3′ end, were designed. Herein it is demonstrated that the addition of Mg2+ ions leads to assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation identified the best‐performing system. The results highlight key features for efficient control of DNAzyme activity through the formation of boronate linkages.
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| Author: | Mégane Debiais, Amandine Lelievre, Jean‐Jacques Vasseur, Sabine Müller, Michael Smietana |
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| URN: | urn:nbn:de:gbv:9-opus-43327 |
| DOI: | https://doi.org/10.1002/chem.202004227 |
| Parent Title (English): | Chemistry – A European Journal |
| Document Type: | Article |
| Language: | English |
| Date of first Publication: | 2021/01/14 |
| Release Date: | 2021/06/02 |
| Tag: | DNA; RNA; autoligation; boronic acids; oligonucleotides |
| GND Keyword: | - |
| Volume: | 27 |
| Issue: | 3 |
| First Page: | 1138 |
| Last Page: | 1144 |
| Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie |
| Licence (German): |  Creative Commons - Namensnennung |