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Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-65267

A Neutrophil Proteomic Signature in Surgical Trauma Wounds

  • on-healing wounds continue to be a clinical challenge for patients and medical staff. These wounds have a heterogeneous etiology, including diabetes and surgical trauma wounds. It is therefore important to decipher molecular signatures that reflect the macroscopic process of wound healing. To this end, we collected wound sponge dressings routinely used in vacuum assisted therapy after surgical trauma to generate wound-derived protein profiles via global mass spectrometry. We confidently identified 311 proteins in exudates. Among them were expected targets belonging to the immunoglobulin superfamily, complement, and skin-derived proteins, such as keratins. Next to several S100 proteins, chaperones, heat shock proteins, and immune modulators, the exudates presented a number of redox proteins as well as a discrete neutrophil proteomic signature, including for example cathepsin G, elastase, myeloperoxidase, CD66c, and lipocalin 2. We mapped over 200 post-translational modifications (PTMs; cysteine/methionine oxidation, tyrosine nitration, cysteine trioxidation) to the proteomic profile, for example, in peroxiredoxin 1. Investigating manually collected exudates, we confirmed presence of neutrophils and their products, such as microparticles and fragments containing myeloperoxidase and DNA. These data confirmed known and identified less known wound proteins and their PTMs, which may serve as resource for future studies on human wound healing

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Metadaten
Author: Sander Bekeschus, Jan-Wilm Lackmann, Denis Gümbel, Matthias Napp, Anke Schmidt, Kristian Wende
URN:urn:nbn:de:gbv:9-opus-65267
DOI:https://doi.org/10.3390/ijms19030761
ISSN:1422-0067
Parent Title (English):International Journal of Molecular Sciences
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of first Publication:2018/03/07
Release Date:2022/11/25
Tag:chaperones; damage-associated molecular patterns; heat-shock proteins; mass spectrometry; matrix metalloproteinase; peptidases; post-translational modifications; redox regulation
GND Keyword:-
Volume:19
Issue:3
Page Number:23
Faculties:Universitätsmedizin / Klinik und Poliklinik für Chirurgie Abt. für Unfall- und Wiederherstellungschirurgie
Licence (German):License LogoCreative Commons - Namensnennung