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Spezifisch bindende magnetische Nanopartikel in magnetischen Relaxationsmessungen

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  • The aim of this study was to show the suitability of a new SQUID-based technique, called magnetorelaxometry, for the specific detection of binding reactions in vitro and its potential in vivo. Magnetic nanoparticles (MNPs) modified with biologically active molecules are utilised as probes. The method is based on the magnetisation of MNPs in a magnetic field and the following detection of their relaxing magnetisation. The specificity of the method depends on the difference of the magnetic relaxation mechanism of MNPs in a liquid phase and immobilised particles. In a liquid phase the particles relax as whole in the surrounding fluid (brownian relaxation). Is the mobility of the magnetic nanoparticles reduced, the magnetisation of the sample decays via the Néel relaxation mechanism. A preparation method for streptavidin- and antibody-conjugated MNPs was developed. Binding reactions in homogeneous and heterogeneous phases were studied. Therefore solid-and liquid-phase immunoassays, magnetic relaxation immunoassays (MARIAs), were established and compared to well-established ELISAs and particles size measurements. Magnetic nanoparticle relaxation measurements provided an increased detection limit compared to particle size measurements and a slightly reduced detection limit compared to ELISA. The in vivo applicability of the method was proven. Furthermore a new magneto-optical in vitro method was developed, that is based on the measurement of the relaxation of the birefringence of magnetised MNPs.

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Metadaten
Author: Julia Lange
URN:urn:nbn:de:gbv:9-200106-3
Title Additional (English):Biologically active magnetic nanoparticles as signal generators in magnetic relaxation measurements
Advisor:Prof. Dr. Werner Weitschies
Document Type:Doctoral Thesis
Language:German
Date of Publication (online):2006/07/12
Granting Institution:Ernst-Moritz-Arndt-Universität, Mathematisch-Naturwissenschaftliche Fakultät (bis 31.05.2018)
Date of final exam:2001/03/26
Release Date:2006/07/12
Tag:Relaxationsmessung, Signalgeber
Brown-Relaxation, Magnetic Relaxation Immnoassay, Magnetic nanoparticles, Magnetorelaxometry, Photon Correlation Spetroscopy
GND Keyword:Magnet, Nanopartikel, Technologie
Faculties:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Pharmazie
DDC class:500 Naturwissenschaften und Mathematik / 540 Chemie