Volltext-Downloads (blau) und Frontdoor-Views (grau)

Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-41747

Axenic in vitro cultivation of 19 peat moss (Sphagnum L.) species as a resource for basic biology, biotechnology, and paludiculture

  • Summary Sphagnum farming can substitute peat with renewable biomass and thus help mitigate climate change. Large volumes of the required founder material can only be supplied sustainably by axenic cultivation in bioreactors. We established axenic in vitro cultures from sporophytes of 19 Sphagnum species collected in Austria, Germany, Latvia, the Netherlands, Russia, and Sweden: S. angustifolium, S. balticum, S. capillifolium, S. centrale, S. compactum, S. cuspidatum, S. fallax, S. fimbriatum, S. fuscum, S. lindbergii, S. medium/divinum, S. palustre, S. papillosum, S. rubellum, S. russowii, S. squarrosum, S. subnitens, S. subfulvum and S. warnstorfii. These species cover five of the six European Sphagnum subgenera; namely, Acutifolia, Cuspidata, Rigida, Sphagnum and Squarrosa. Their growth was measured in suspension cultures, whereas their ploidy was determined by flow cytometry and compared with the genome size of Physcomitrella patens. We identified haploid and diploid Sphagnum species, found that their cells are predominantly arrested in the G1 phase of the cell cycle, and did not find a correlation between plant productivity and ploidy. DNA barcoding was achieved by sequencing introns of the BRK1 genes. With this collection, high‐quality founder material for diverse large‐scale applications, but also for basic Sphagnum research, is available from the International Moss Stock Center.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar


Author: Melanie A. Heck, Volker M. Lüth, Nico van Gessel, Matthias Krebs, Mira Kohl, Anja Prager, Hans Joosten, Eva L. Decker, Ralf Reski
Parent Title (English):New Phytologist
Place of publication:Hoboken, New Jersey
Document Type:Article
Date of first Publication:2020/10/07
Release Date:2021/02/10
Tag:DNA barcoding; cell cycle arrest; climate change; farming; genome size; peat moss; peatland restoration
GND Keyword:-
First Page:n/a
Last Page:n/a
Faculties:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Botanik und Landschaftsökologie & Botanischer Garten
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung