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Boronic Acid‐Mediated Activity Control of Split 10–23 DNAzymes

  • Abstract The 10–23 DNAzyme is an artificially developed Mg2+‐dependent catalytic oligonucleotide that can cleave an RNA substrate in a sequence‐specific fashion. In this study, new split 10–23 DNAzymes made of two nonfunctional fragments, one of which carries a boronic acid group at its 5′ end, while the other has a ribonucleotide at its 3′ end, were designed. Herein it is demonstrated that the addition of Mg2+ ions leads to assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation identified the best‐performing system. The results highlight key features for efficient control of DNAzyme activity through the formation of boronate linkages.

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Author: Mégane Debiais, Amandine Lelievre, Jean‐Jacques Vasseur, Sabine Müller, Michael Smietana
Parent Title (English):Chemistry – A European Journal
Document Type:Article
Date of first Publication:2021/01/14
Release Date:2021/06/02
Tag:DNA; RNA; autoligation; boronic acids; oligonucleotides
GND Keyword:-
First Page:1138
Last Page:1144
Faculties:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie und Biochemie
Licence (German):License LogoCreative Commons - Namensnennung