Volltext-Downloads (blau) und Frontdoor-Views (grau)

Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-56881

Analysis and Reduction of Cellular Heterogeneity in Strain Optimization of Bacillus licheniformis

  • Bacillus species invest substantial resources in inherent cellular processes for pre-adaptation to environmental changes, many of which are dispensable in the controlled environment of industrial bioprocesses. The underlying physiological mechanisms are well characterized in B. subtilis, but only little is known about these processes in the closely related B. licheniformis. Moreover, experimental conditions in previous studies differ from industrial settings in most parameters, foremost in batch cultures or plate-based analysis over fed-batch processes. In this thesis, cellular heterogeneity was analyzed in B. licheniformis in optimized, nutrient-rich media in batch and fed-batch cultivations. Systematic inactivation of genes involved in biofilm formation and synthesis of the flagellar apparatus or global regulators thereof resulted in higher protein production and provided new insights into biofilm formation and cellular heterogeneity in this strain.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar


Author: Mathis Appelbaum
Title Additional (German):Analyse und Reduktion der zellulären Heterogenität im Kontext der Stammoptimierung von Bacillus licheniformis
Referee:Prof. Dr. Thomas Schweder, Prof. Dr. Erhard Bremer, Prof. Dr. Peter Neubauer
Advisor:Prof. Dr. Thomas Schweder
Document Type:Doctoral Thesis
Year of Completion:2021
Date of first Publication:2021/10/20
Granting Institution:Universität Greifswald, Mathematisch-Naturwissenschaftliche Fakultät
Date of final exam:2021/09/29
Release Date:2021/10/20
Tag:Zellheterogenität; Zelluläre Heterogenität
GND Keyword:Bacillus
Page Number:189
Faculties:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Pharmazie
DDC class:500 Naturwissenschaften und Mathematik / 570 Biowissenschaften; Biologie