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This study evaluated the impact of a defined plasma treated water (PTW) when applied to various stages within fresh-cut endive processing. The quality characteristic responses were investigated to establish the impact of the PTW unit processes and where PTW may be optimally applied in a model process line to retain or improve produce quality. Different stages of application of PTW within the washing process were investigated and compared to tap water and chlorine dioxide. Fresh-cut endive (Cichorium endivia L.) samples were analyzed for retention of food quality characteristics. Measurements included color, texture, and nitrate quantification. Effects on tissue surface and cell organelles were observed through scanning electron and atomic force microscopy. Overall, the endive quality characteristics were retained by incorporating PTW in the washing process. Furthermore, promising results for color and texture characteristics were observed, which were supported by the microscopic assays of the vegetal tissue. While ion chromatography detected high concentrations of nitrite and nitrate in PTW, these did not affect the nitrate concentration of the lettuce tissue post-processing and were below the concentrations within EU regulations. These results provide a pathway to scale up the industrial application of PTW to improve and retain quality characteristic retention of fresh leafy products, whilst also harnessing the plasma functionalized water as a process intervention for reducing microbial load at multiple points, whether on the food surface, within the process water or on food-processing surfaces.
Background: Plasma-generated compounds (PGCs) such as plasma-processed air (PPA) or plasma-treated water (PTW) offer an increasingly important alternative for the control of microorganisms in hard-to-reach areas found in several industrial applications including the food industry. To this end, we studied the antimicrobial capacity of PTW on the vitality and biofilm formation of Listeria monocytogenes, a common foodborne pathogen.
Results: Using a microwave plasma (MidiPLexc), 10 ml of deionized water was treated for 100, 300, and 900 s (pre-treatment time), after which the bacterial biofilm was exposed to the PTW for 1, 3, and 5 min (post-treatment time) for each pre-treatment time, separately. Colony-forming units (CFU) were significantly reduced by 4.7 log10 ± 0.29 log10, as well as the metabolic activity decreased by 47.9 ± 9.47% and the cell vitality by 69.5 ± 2.1%, compared to the control biofilms. LIVE/DEAD staining and fluorescence microscopy showed a positive correlation between treatment and incubation times, as well as reduction in vitality. Atomic force microscopy (AFM) indicated changes in the structure quality of the bacterial biofilm.
Conclusion: These results indicate a promising antimicrobial impact of plasma-treated water on Listeria monocytogenes, which may lead to more targeted applications of plasma decontamination in the food industry in the future.
Die vorliegende Arbeit widmet sich der plasmachemischen Herstellung und physikalisch-chemischen Charakterisierung von dünnen organischen Schichten auf der Basis von Ethylenglykol (Präkursor). Die Oberflächen können die Adsorption von Proteinen minimieren und daher als neues biokompatibles Material getestet werden. Im Schwerpunkt der Arbeit liegt die Entwicklung eines plasmachemischen RF-Reaktors (genannt Nevada) und einer innovativen Beschichtungstechnologie TFPD (Temperature Forced Plasma Deposition) als Erweiterung der PECVD (Plasma Enhanced Chemical Vapour Deposition). Ein Gemisch aus Ethylenglykol und Argon wurde als Modellsystem untersucht. Die Plasmabedingungen und die Oberflächentemperatur wurden optimiert und an Phaseneigenschaften von Ethylenglykol angepasst. Die komplexe Polykondensation steht für die plasmagestützte Umwandlung der Kondensatschicht zu einem stabilen Poly(Ethylenglykol)-ähnlichen Plasmapolymer. Der Prozess wurde durch die simultane Temperaturkontrolle und in situ FTIR-Spektroskopie analysiert. Zur Untersuchung der erzeugten Proben wurden weitere ex situ Oberflächenanalysen wie XPS, AFM, TDS, MALDI, XRD und die optische Ellipsometrie verwendet. Durch die neue Methode TFPD entstehen extrem glatte, amorphe und wasserunlösliche Schichten mit einem Potenzial für die Biokompatibilität.