Refine
Document Type
- Article (2)
Language
- English (2) (remove)
Has Fulltext
- yes (2)
Is part of the Bibliography
- no (2)
Keywords
- - (2)
- actin cytoskeleton (1)
- actin quantification (1)
- amino polymer (1)
- calcium ion signaling (1)
- cell spreading (1)
- cell-material interaction (1)
- human osteoblasts (1)
- mathematical modeling (1)
- osteoblasts (1)
- polyelectrolyte multilayer (1)
- scanning ion conductance microscopy (1)
- surface charge (1)
- surface charge sensing (1)
- titanium surface modification (1)
- wettability (1)
- zeta potential (1)
Institute
- Institut für Physik (2) (remove)
Publisher
- Frontiers Media S.A. (1)
- MDPI (1)
Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between −90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (−90 to −3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials’ zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.