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Decreased inflammatory profile in oral leukoplakia tissue exposed to cold physical plasma ex vivo
- Background Oral leukoplakia (OL) is an unfavorable oral disease often resistant to therapy. To this end, cold physical plasma technology was explored as a novel therapeutic agent in an experimental setup. Methods Biopsies with a diameter of 3 mm were obtained from non-diseased and OL tissues. Subsequently, cold atmospheric pressure plasma (CAP) exposure was performed ex vivo in the laboratory. After 20 h of incubation, biopsies were cryo-conserved, and tissue sections were quantified for lymphocyte infiltrates, discriminating between naïve and memory cytotoxic and T-helper cells. In addition, the secretion pattern related to inflammation was investigated in the tissue culture supernatants by quantifying 10 chemokines and cytokines. Results In CAP-treated OL tissue, significantly decreased overall lymphocyte numbers were observed. In addition, reduced levels were observed when discriminating for the T-cell subpopulations but did not reach statistical significance. Moreover, CAP treatment significantly reduced levels of C-X-C motif chemokine 10 (CXCL10) and granulocyte-macrophage colony-stimulating factor in the OL biopsies' supernatants. In idiopathically inflamed tissues, ex vivo CAP exposure reduced T-cells and CXCL10 as well but also led to markedly increased interleukin-1β secretion. Conclusion Our findings suggest CAP to have immuno-modulatory properties, which could be of therapeutic significance in the therapy of OL. Future studies should investigate the efficacy of CAP therapy in vivo in a larger cohort.
Author: | Christian Seebauer, Eric FreundORCiD, Tobias Dieke, Sybille Hasse, Maria Segebarth, Christoph Rautenberg, Hans-Robert Metelmann, Sander BekeschusORCiD |
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URN: | urn:nbn:de:gbv:9-opus-109409 |
DOI: | https://doi.org/10.1111/jop.13496 |
ISSN: | 1600-0714 |
Parent Title (English): | Journal of Oral Pathology & Medicine |
Publisher: | Wiley |
Place of publication: | Hoboken, NJ |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2023/10/12 |
Date of first Publication: | 2023/11/01 |
Release Date: | 2024/03/27 |
Tag: | T-cells; inflammation; kINPen MED; mucosal diseases |
Volume: | 52 |
Issue: | 10 |
First Page: | 1021 |
Last Page: | 1028 |
Faculties: | Universitätsmedizin / Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie/Plastische Operationen |
Collections: | weitere DFG-förderfähige Artikel |
Licence (German): | Creative Commons - Namensnennung 4.0 International |