Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-40024
Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges
- Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between −90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (−90 to −3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials’ zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.
Author: | Martina Gruening, Sven Neuber, Peter Nestler, Jutta Lehnfeld, Manuela Dubs, Katja Fricke, Matthias Schnabelrauch, Christiane A. Helm, Rainer Müller, Susanne Staehlke, J. Barbara Nebe |
---|---|
URN: | urn:nbn:de:gbv:9-opus-40024 |
DOI: | https://doi.org/10.3389/fbioe.2020.01016 |
ISSN: | 2296-4185 |
Parent Title (English): | Frontiers in Bioengineering and Biotechnology |
Publisher: | Frontiers Media S.A. |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2020/09/08 |
Release Date: | 2020/12/01 |
Tag: | amino polymer; calcium ion signaling; human osteoblasts; polyelectrolyte multilayer; surface charge; titanium surface modification; wettability; zeta potential |
GND Keyword: | - |
Volume: | 8 |
Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik |
Licence (German): | Creative Commons - Namensnennung |