Bitte verwenden Sie diesen Link, wenn Sie dieses Dokument zitieren oder verlinken wollen: https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-64036
Molecular and Biochemical Differences of the Tandem and Cold-Adapted PET Hydrolases Ple628 and Ple629, Isolated From a Marine Microbial Consortium
- Polybutylene adipate terephthalate (PBAT) is a biodegradable alternative to polyethylene and can be broadly used in various applications. These polymers can be degraded by hydrolases of terrestrial and aquatic origin. In a previous study, we identified tandem PETase-like hydrolases (Ples) from the marine microbial consortium I1 that were highly expressed when a PBAT blend was supplied as the only carbon source. In this study, the tandem Ples, Ple628 and Ple629, were recombinantly expressed and characterized. Both enzymes are mesophilic and active on a wide range of oligomers. The activities of the Ples differed greatly when model substrates, PBAT-modified polymers or PET nanoparticles were supplied. Ple629 was always more active than Ple628. Crystal structures of Ple628 and Ple629 revealed a structural similarity to other PETases and can be classified as member of the PETases IIa subclass, α/β hydrolase superfamily. Our results show that the predicted functions of Ple628 and Ple629 agree with the bioinformatic predictions, and these enzymes play a significant role in the plastic degradation by the consortium.
Author: | Ingrid E. Meyer Cifuentes, Pan Wu, Yipei Zhao, Weidong Liu, Meina Neumann-Schaal, Lara Pfaff, Justyna Barys, Zhishuai Li, Jian Gao, Xu Han, Uwe T. BornscheuerORCiD, Ren Wei, Başak Öztürk |
---|---|
URN: | urn:nbn:de:gbv:9-opus-64036 |
DOI: | https://doi.org/10.3389/fbioe.2022.930140 |
ISSN: | 2296-4185 |
Parent Title (English): | Frontiers in Bioengineering and Biotechnology |
Publisher: | Frontiers Media S.A. |
Place of publication: | Lausanne |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2022/07/21 |
Release Date: | 2022/11/25 |
Tag: | PETase activity; PETase-like enzymes; biodegradable plastics; marine biodegradation; tandem PETases |
GND Keyword: | - |
Volume: | 10 |
Article Number: | 930140 |
Page Number: | 13 |
Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie |
Licence (German): | Creative Commons - Namensnennung |