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GMP-Compliant Manufacturing of TRUCKs: CAR T Cells targeting GD2 and Releasing Inducible IL-18
- Chimeric antigen receptor (CAR)-engineered T cells can be highly effective in the treatment of hematological malignancies, but mostly fail in the treatment of solid tumors. Thus, approaches using 4th advanced CAR T cells secreting immunomodulatory cytokines upon CAR signaling, known as TRUCKs (“T cells redirected for universal cytokine-mediated killing”), are currently under investigation. Based on our previous development and validation of automated and closed processing for GMP-compliant manufacturing of CAR T cells, we here present the proof of feasibility for translation of this method to TRUCKs. We generated IL-18-secreting TRUCKs targeting the tumor antigen GD2 using the CliniMACS Prodigy® system using a recently described “all-in-one” lentiviral vector combining constitutive anti-GD2 CAR expression and inducible IL-18. Starting with 0.84 x 108 and 0.91 x 108 T cells after enrichment of CD4+ and CD8+ we reached 68.3-fold and 71.4-fold T cell expansion rates, respectively, in two independent runs. Transduction efficiencies of 77.7% and 55.1% was obtained, and yields of 4.5 x 109 and 3.6 x 109 engineered T cells from the two donors, respectively, within 12 days. Preclinical characterization demonstrated antigen-specific GD2-CAR mediated activation after co-cultivation with GD2-expressing target cells. The functional capacities of the clinical-scale manufactured TRUCKs were similar to TRUCKs generated in laboratory-scale and were not impeded by cryopreservation. IL-18 TRUCKs were activated in an antigen-specific manner by co-cultivation with GD2-expressing target cells indicated by an increased expression of activation markers (e.g. CD25, CD69) on both CD4+ and CD8+ T cells and an enhanced release of pro-inflammatory cytokines and cytolytic mediators (e.g. IL-2, granzyme B, IFN-γ, perforin, TNF-α). Manufactured TRUCKs showed a specific cytotoxicity towards GD2-expressing target cells indicated by lactate dehydrogenase (LDH) release, a decrease of target cell numbers, microscopic detection of cytotoxic clusters and detachment of target cells in real-time impedance measurements (xCELLigence). Following antigen-specific CAR activation of TRUCKs, CAR-triggered release IL-18 was induced, and the cytokine was biologically active, as demonstrated in migration assays revealing specific attraction of monocytes and NK cells by supernatants of TRUCKs co-cultured with GD2-expressing target cells. In conclusion, GMP-compliant manufacturing of TRUCKs is feasible and delivers high quality T cell products.
Author: | Wolfgang Glienke, Anna Christina Dragon, Katharina Zimmermann, Alexandra Martyniszyn-Eiben, Mira Mertens, Hinrich Abken, Claudia Rossig, Bianca Altvater, Krasimira Aleksandrova, Lubomir Arseniev, Christina Kloth, Andriana Stamopoulou, Thomas Moritz, Holger N. LodeORCiD, Nikolai Siebert, Rainer Blasczyk, Lilia Goudeva, Axel Schambach, Ulrike Köhl, Britta Eiz-Vesper, Ruth Esser |
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URN: | urn:nbn:de:gbv:9-opus-61316 |
DOI: | https://doi.org/10.3389/fimmu.2022.839783 |
ISSN: | 1664-3224 |
Parent Title (English): | Frontiers in Immunology |
Publisher: | Frontiers Media S.A. |
Place of publication: | Lausanne |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2022/03/24 |
Release Date: | 2022/11/15 |
Tag: | 4; GD2-CAR; GMP; IL-18; Prodigy; TRUCK |
GND Keyword: | - |
Volume: | 13 |
Article Number: | 839783 |
Page Number: | 17 |
Faculties: | Universitätsmedizin / Kliniken und Polikliniken für Innere Medizin |
Licence (German): | Creative Commons - Namensnennung |