Doctoral Thesis
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Scholz et al. developed an electrochemical assay to study the impact of reactive species on self-assembled monolayer (SAM). The aim of this thesis is to use this electrochemical assay with gold supported lipid bilayers instead of SAM to study the effect of reactive species on model membranes that mimic oxidative damage to the biological cell membrane. Here, three questions will be addressed: I) how specific substances such as lipophilic and hydrophilic antioxidants protect a membrane from oxidative damage, II) what are the lipid oxidation products after oxidative damage of the model membrane, and III) whether oxidative damage of the model membranes causes pore formation on lipid bilayer. Electrochemistry was first used to measure the oxidative damage over the entire lipid membrane. Then, mass spectroscopy was used to characterize how lipids as the molecular building blocks of the membrane, change when exposed to reactive species. Imaging the membrane with AFM showed how oxidative damage in the model membrane alters lipid self-assembly within the supported lipid bilayer in nanometer scale. In addition, cold physical plasma (CPP) was used to produce the biological relevant reactive species. This fundamental research demonstrates the great potential of supported lipid bilayers as model membranes and cold physical plasma as a source for the production of biologically relevant reactive species to study the effect of oxidative stress on cell membranes.