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Ex vivo anticoagulants affect human blood platelet biomechanics with implications for high-throughput functional mechanophenotyping
- Inherited platelet disorders affecting the human platelet cytoskeleton result in increased bleeding risk. However, deciphering their impact on cytoskeleton-dependent intrinsic biomechanics of platelets remains challenging and represents an unmet need from a diagnostic and prognostic perspective. It is currently unclear whether ex vivo anticoagulants used during collection of peripheral blood impact the mechanophenotype of cellular components of blood. Using unbiased, high-throughput functional mechanophenotyping of single human platelets by real-time deformability cytometry, we found that ex vivo anticoagulants are a critical pre-analytical variable that differentially influences platelet deformation, their size, and functional response to agonists by altering the cytoskeleton. We applied our findings to characterize the functional mechanophenotype of platelets from a patient with Myosin Heavy Chain 9 (MYH9) related macrothrombocytopenia. Our data suggest that platelets from MYH9 p.E1841K mutation in humans affecting platelet non-muscle myosin heavy chain IIa (NMMHC-IIA) are biomechanically less deformable in comparison to platelets from healthy individuals.
Author: | Laura Sachs, Jan Wesche, Lea Lenkeit, Andreas GreinacherORCiD, Markus Bender, Oliver Otto, Raghavendra Palankar |
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URN: | urn:nbn:de:gbv:9-opus-107490 |
DOI: | https://doi.org/10.1038/s42003-021-02982-6 |
ISSN: | 2399-3642 |
Parent Title (English): | Communications Biology |
Publisher: | Nature Publishing Group |
Place of publication: | London |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2022/01/21 |
Release Date: | 2024/02/26 |
Volume: | 5 |
Article Number: | 86 |
Page Number: | 14 |
Faculties: | Universitätsmedizin / Institut für Immunologie u. Transfusionsmedizin - Abteilung Transfusionsmedizin |
Collections: | weitere DFG-förderfähige Artikel |
Licence (German): | Creative Commons - Namensnennung 4.0 International |