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Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models
- As a new field of oxidative stress-based therapy, cold physical plasma is a promising tool for several biomedical applications due to its potential to create a broad diversity of reactive oxygen and nitrogen species (RONS). Although proposed, the impact of plasma-derived RONS on the cell membrane lipids and properties is not fully understood. For this purpose, the changes in the lipid bilayer functionality under oxidative stress generated by an argon plasma jet (kINPen) were investigated by electrochemical techniques. In addition, liquid chromatography-tandem mass spectrometry was employed to analyze the plasma-induced modifications on the model lipids. Various asymmetric bilayers mimicking the structure and properties of the erythrocyte cell membrane were transferred onto a gold electrode surface by Langmuir-Blodgett/Langmuir-Schaefer deposition techniques. A strong impact of cholesterol on membrane permeabilization by plasma-derived species was revealed. Moreover, the maintenance of the barrier properties is influenced by the chemical composition of the head group. Mainly the head group size and its hydrogen bonding capacities are relevant, and phosphatidylcholines are significantly more susceptible than phosphatidylserines and other lipid classes, underlining the high relevance of this lipid class in membrane dynamics and cell physiology.
Author: | Zahra Nasri, Mohsen Ahmadi, Johanna Striesow, Mehdi Ravandeh, Thomas von Woedtke, Kristian Wende |
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URN: | urn:nbn:de:gbv:9-opus-64475 |
DOI: | https://doi.org/10.3390/ijms23115932 |
ISSN: | 1422-0067 |
Parent Title (English): | International Journal of Molecular Sciences |
Publisher: | MDPI |
Place of publication: | Basel |
Editor: | Felipe Martinez-Pastor |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2022/05/25 |
Release Date: | 2022/11/15 |
Tag: | Langmuir-Blodgett and Schaefer techniques; cell membrane; cold physical plasma; electrochemistry; mass spectrometry; reactive oxygen and nitrogen species; supported lipid bilayers |
GND Keyword: | - |
Volume: | 23 |
Issue: | 11 |
Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik |
Licence (German): | Creative Commons - Namensnennung |