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MiR-502 is the first reported miRNA simultaneously targeting two components of the classical non-homologous end joining (C-NHEJ) in pancreatic cell lines.
- Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Acquired inherited and/or somaticmutations drive its development. In order to prevent the formation of these mutations, precise and immediaterepair of any DNA damage is indispensable. Non-homologous end-joining (NHEJ) is the key mechanism of DNAdouble-strand break repair. Here, we report that miR-502 targets two components in pancreatic cell lines, Ku70and XLF of the C-NHEJ. Interestingly, we also observed an attenuated cell cycle response to gamma ionizingradiation (γ-IR) via diminished phosphorylation of checkpoint kinase 1 (Chk1) on serine 345 in these cell lines.Altogether, pancreatic cells showed increased susceptibility toγ-IR via direct inhibition of DNA double-strandbreak repair and attenuation of the cell cycle response.
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| Author: | Agnieszka Smolinska, Julia Swoboda, Wojciech Fendler, Markus M. Lerch, Matthias Sendler, Patryk MoskwaORCiD |
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| URN: | urn:nbn:de:gbv:9-opus-43880 |
| DOI: | https://doi.org/https://doi.org/10.1016/j.heliyon.2020.e03187 |
| ISSN: | 2405-8440 |
| Parent Title (English): | Heliyon |
| Publisher: | Elsevier |
| Place of publication: | London |
| Document Type: | Article |
| Language: | English |
| Date of first Publication: | 2020/01/06 |
| Release Date: | 2021/03/10 |
| Tag: | Cell culture; Cell death; Chemotherapy; DNA epair; DNA repair; DSB; Gene mutation; PDAC; miRNA |
| Volume: | 6 |
| Issue: | 1 |
| Page Number: | 9 |
| Faculties: | Universitätsmedizin / Klinik und Poliklinik für Innere Medizin |
| Collections: | Artikel aus DFG-gefördertem Publikationsfonds |
| Licence (German): |  Creative Commons - Namensnennung |