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MiR-502 is the first reported miRNA simultaneously targeting two components of the classical non-homologous end joining (C-NHEJ) in pancreatic cell lines.

  • Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Acquired inherited and/or somaticmutations drive its development. In order to prevent the formation of these mutations, precise and immediaterepair of any DNA damage is indispensable. Non-homologous end-joining (NHEJ) is the key mechanism of DNAdouble-strand break repair. Here, we report that miR-502 targets two components in pancreatic cell lines, Ku70and XLF of the C-NHEJ. Interestingly, we also observed an attenuated cell cycle response to gamma ionizingradiation (γ-IR) via diminished phosphorylation of checkpoint kinase 1 (Chk1) on serine 345 in these cell lines.Altogether, pancreatic cells showed increased susceptibility toγ-IR via direct inhibition of DNA double-strandbreak repair and attenuation of the cell cycle response.
Author: Agnieszka Smolinska, Julia Swoboda, Wojciech Fendler, Markus M. LerchORCiD, Matthias SendlerORCiD, Patryk MoskwaORCiD
Parent Title (English):Heliyon
Place of publication:London
Document Type:Article
Date of first Publication:2020/01/06
Release Date:2021/03/10
Tag:Cell culture; Cell death; Chemotherapy; DNA epair; DNA repair; DSB; Gene mutation; PDAC; miRNA
Page Number:9
Faculties:Universitätsmedizin / Kliniken und Polikliniken für Innere Medizin
Collections:Artikel aus DFG-gefördertem Publikationsfonds
Licence (German):License LogoCreative Commons - Namensnennung