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Application and elucidation of enzymes involved in the utilization of marine carbohydrates
- This dissertation focuses on the characterization of novel enzymes and metabolic pathways that fulfill crucial functions during marine carbohydrate degradation by Bacteroidetes and thus contributes to an advanced understanding of the global carbon cycle. Depolymerization and utilization of marine polysaccharides by Bacteroidetes requires a tremendous repertoire of enzymes with a wide range of functions. For instance, during the breakdown of the marine red algal polysaccharide porphyran, an oxidative demethylation of the methoxy sugar 6-O-methyl-D-galactose (G6Me) by cytochrome P450 monooxygenases occurs. This reaction produces huge amounts of cytotoxic formaldehyde, marine bacteria capable of degrading porphyran must therefore possess suitable formaldehyde detoxification pathways. Consequently, Article I focus on the identification of possible formaldehyde detoxification pathways in marine Flavobacteriia, which led to the discovery of the ribulose monophosphate pathway as specific pathway for the detoxification of formaldehyde in certain Bacteroidetes like Zobellia galactanivorans. Furthermore, it was demonstrated in Article II that alcohol dehydrogenases play an essential role in the microbial utilization of G6Me and therefore possess a function in porphyran degradation. Discovering novel enzymes, entire enzymatic cascades or biotechnologically important microorganisms that can metabolize these marine carbohydrates also contributes to the utilization of marine polysaccharides as feedstock for potential biotechnological applications. A prospective biorefinery process was proposed in Article III by the identification of Bacillus licheniformis as promising utilizer of marine carbohydrate-derived monosaccharides and the creation of a microbial cell factory capable of growing on ulvan, a marine carbohydrate obtainable from algal bloom-dominating green algae, enabling an industrial use of the renewable and abundant algal biomass in future.
Author: | Stefan Brott |
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URN: | urn:nbn:de:gbv:9-opus-103965 |
Title Additional (German): | Anwendung und Identifizierung von Enzymen, die in der Verwertung mariner Kohlenhydrate involviert sind |
Referee: | Prof. Dr. Uwe BornscheuerORCiD, Prof. Dr. Magali Remaud-Simeon |
Advisor: | Prof. Dr. Uwe Bornscheuer |
Document Type: | Doctoral Thesis |
Language: | English |
Year of Completion: | 2023 |
Date of first Publication: | 2023/12/07 |
Granting Institution: | Universität Greifswald, Mathematisch-Naturwissenschaftliche Fakultät |
Date of final exam: | 2023/11/30 |
Release Date: | 2023/12/07 |
Tag: | algae; enzyme Bacteroidetes; formaldehyde; polysaccharide |
GND Keyword: | algae, polysaccharide, Bacteroidetes, formaldehyde |
Page Number: | 171 |
Faculties: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie |
DDC class: | 500 Naturwissenschaften und Mathematik / 500 Naturwissenschaften |